Antigen identification strategies and preclinical evaluation models for advancing tuberculosis vaccine development

In its myriad devastating forms, Tuberculosis (TB) has existed for centuries, and humanity is still affected by it. Mycobacterium tuberculosis (M. tuberculosis), the causative agent of TB, was the foremost killer among infectious agents until the COVID-19 pandemic. One of the key healthcare strategies available to reduce the risk of TB is immunization with bacilli Calmette-Guerin (BCG). Although BCG has been widely used to protect against TB, reports show that BCG confers highly variable efficacy (0-80%) against adult pulmonary TB. Unwavering efforts have been made over the past 20 years to develop and evaluate new TB vaccine candidates. The failure of conventional preclinical animal models to fully recapitulate human response to TB, as also seen for the failure of MVA85A in clinical trials, signifies the need to develop better preclinical models for TB vaccine evaluation. In the present review article, we outline various approaches used to identify protective mycobacterial antigens and recent advancements in preclinical models for assessing the efficacy of candidate TB vaccines.

Tuberculosis (TB) remains a major global health concern that accounts for nearly 4000 lives daily.Bacilli Calmette-Guerin (BCG), the only licensed and century-old vaccine, has conferred variable protection in several field trials [1][2][3][4] .Developing better vaccines has been a crucial objective in TB research.The decoding of the complete genome of M. tuberculosis in 1998 represents a breakthrough in TB vaccine research and has resulted in significant advances in the area of TB vaccine antigen discovery 5 .Several different approaches have been used to identify antigens for developing TB vaccines over the past two decades.Identifying M. tuberculosis antigens and epitopes has enhanced our knowledge of the M. tuberculosis antigenome, leading to the identification of various candidates for TB vaccine development.Antigen discovery for vaccine development relies on identifying immunogenic proteins of M. tuberculosis, and many antigens have been identified using conventional and genome-wide screening approaches.While Th1 responses are generally acknowledged to be crucial for protection against M. tuberculosis, a TB vaccine that induced antigen-specific CD4 T-cells did not show any efficacy against M. tuberculosis infection in an infant efficacy trial 6 .New antigens that can activate multiple immune components, including the non-classical T-cell and antibody responses, are important for vaccine development.Recent studies have demonstrated that antibodies against different surface antigens are able to impart moderate protection against M. tuberculosis in preclinical animal models [7][8][9][10] .Preclinical evaluation studies in non-human primates indicate that multiantigenic vectored vaccines, which combine latency antigens with classical early secreted antigens and resuscitation promoting factors of M. tuberculosis, have a strong vaccine potential.Immunization of rhesus macaques with this cytomegalovirus-based vaccine was able to impart unprecedented protection against TB disease 11 .Multi-antigenic vectored vaccination strategies have also demonstrated potential as immunotherapeutics in postchallenge mouse models 12 .The preclinical success of two distinct vaccine candidates in achieving sterilizing immunity in a proportion of animals shows that an effective TB vaccine is feasible and may elicit unique immune mechanisms of protection 11,13 .The major hindrance in developing new TB vaccines is a lack of (i) understanding of how antigens are recognized by the

Discovery of dormancy antigens and resuscitation promoting factors as promising vaccine target antigens
M. tuberculosis considerably alters its antigenic repertoire during different stages of infection 49 .During in vivo infection, M. tuberculosis multiplication is typically controlled by the host immune system and enters dormancy with reduced or no metabolic activity 50 .In vitro dormancy models capture different aspects of the hypoxic environment encountered by the dormant bacilli in vivo 51,52 .Microarray studies have identified >100 M. tuberculosis genes that are differentially expressed in response to hypoxic conditions 53 .The 16-kDa α-crystallin-like small heat shock protein (HspX/Acr, Rv2031c) is one of the most prominent latency antigens, with its expression markedly increased under oxygenlimiting conditions and in stationary phase cultures of M. tuberculosis 51,54,55 .HspX is required for the intracellular growth of M. tuberculosis in macrophages and is involved in cell wall thickening under hypoxic environments 56 .HspX has demonstrated robust immunogenicity in animal models and humans [57][58][59][60][61] .Based on these observations, HspX has been used as a candidate vaccine antigen in multiple TB vaccine constructs [62][63][64][65][66][67][68][69][70][71][72] .The analysis of the M. tuberculosis transcriptome and proteome in an in vitro nutrient starvation model resulted in the identification of proteins necessary for adaptation to nutrient starvation 73 .Among these starvation-induced proteins, Rv2660, (a conserved hypothetical protein) was the most upregulated protein [73][74][75] .Rv2660c is currently being evaluated in the clinical trial as a component of the multistage TB vaccine candidate H56 (Table 1, Fig. 1) 76 .The H56 vaccine is a protein vaccine that combines the latency-associated antigen Rv2660c, with early antigens Ag85B and ESAT-6 formulated in a Ad5-Ag85A/Viral vectored/ Prime-Boost A replication-deficient serotype 5 Adenovirus vector expressing Ag85A.
Booster of BCG-primed macaques with Ad5-Ag85A reduced lung bacterial loads by at least 100fold compared to the non-vaccinated group..In a pre-exposure mouse model, boosting BCG with H56 resulted in the induction of vaccine-specific polyfunctional CD4 + T-cell response and a significant reduction (~7.0-fold) in lung bacterial load compared to immunization with BCG alone 76 .Vaccination with H56 post-M.tuberculosis exposure protected against latent TB reactivation, and boosting BCG with H56 prevented latent reactivation in cynomolgus macaques 76,77 .To identify human T-cell antigens suitable for subunit TB vaccine development, Bertholet et al. screened 94 M. tuberculosis proteins belonging to either PE/PPE or ESX family or proteins involved in growth in macrophages, hypoxia, secretion, or membrane association for IFN-γ recall responses using PBMCs from healthy subjects exposed to M. tuberculosis 78 .Based on these screening results, 48 antigens were further tested as adjuvanted subunit vaccines in the murine M. tuberculosis aerosol infection model.Three secreted proteins, two PE/PPE proteins, and one hypoxia-associated protein showed >0.3 log 10 reduction in lung CFU compared to shamimmunized mice.Rv1813c (hypoxia-associated), Rv3620 (ESX family), and Rv2608 (PE/PPE family) were combined in a multi-antigen subunit vaccine formulation with CpG adjuvant.Immunization of mice with this multi-antigen subunit vaccine reduced lung bacterial loads compared to adjuvant only control by ∼5.0-fold, and the observed protection was comparable to BCG-vaccinated mice 78 .The same three antigens, Rv1813c, Rv2608, and Rv3620, together with Rv3619, are included as a fusion protein in the subunit vaccine construct ID93 combined with adjuvant GLA-SE and are currently in the TB vaccine trial pipeline 79 (Table 1, Fig. 1).M. tuberculosis induces the expression of an array of latency-associated antigens belonging to the 48-gene-DosR regulon as an adaptive response to dormancy 75,80 .M. tuberculosis expresses the DosR regulon under low oxygen and nitric oxide conditions 80 .Several studies have demonstrated the immunogenicity and vaccine potential of the dormancy regulon-associated antigens 65,[81][82][83][84][85][86] .In a study evaluating human T-cell responses to 25 strongly expressed proteins of the DosR regulon, the majority of the antigens generated strong IFN-γ response among tuberculin skin test (TST) positive individuals with latent TB, in comparison to active TB patients, indicating their potential protective role in infected individuals 86 .Notably, Rv1733c, Rv2029c, Rv2627c, and Rv2628, were predominantly recognized by TSTpositive individuals and have emerged as promising antigenic targets 86 .In concordance, long-term latently infected individuals showed the presence of mono-and polyfunctional cytokine-producing CD4 + and CD8 + T-cells specific for DosR encoded antigens Rv1733c, Rv2029c and Rv2031c 87 .DosR-regulon-encoded antigen Rv2628 was strongly recognized by individuals with a history of TB infection (≥ three years before study enrolment) 88 .Immunization of mice with DNA vaccines encoding Rv2031c (HspX) and Rv2626c induced robust Th1 response, and several T-cell epitopes were mapped on these antigens 65 .Rv1733c encodes a membrane protein and is the most frequently recognized DosR regulon encoded protein in M. tuberculosis exposed household contacts from three high TB burden populations in Africa 82 .Immunization of mice with a synthetic long peptide (SLP) derived from Rv1733c induced a potent T-cell response and improved the efficacy of BCG, resulting in a ~8.0-fold reduction in lung bacillary load compared to BCG-immunized mice (~5.0-fold reduction) 83 .Mice immunized with recombinant BCG expressing Rv1733c along with Rv2659c (latency antigen), Rv3407 (reactivation antigen), and membraneperforating listeriolysin (hly) significantly induced IFN-γ response in splenocytes and imparted 10.0-fold better protection (reduced bacterial burdens in lungs) compared to BCG-immunized mice against M. tuberculosis Beijing/W challenge 84 .The immunomodulatory properties of dormancy antigens as Toll-like receptor (TLR) agonists have also attracted attention as adjuvants for developing subunit vaccines.The use of HspX as a TLR agonist in adjuvant-based tumor immunotherapy has been reported 89 .Recent studies showed that dormancy-associated M. tuberculosis antigens such as Rv2627c, Rv2628, and Rv2659c induce innate and adaptive immune responses through TLR activation 90,91 .The adjuvant-like properties of these antigens could be harnessed for the rational design of multi-epitope subunit vaccines.
Heparin-binding haemagglutinin (HBHA), an extrapulmonary dissemination factor, induces strong cellular immunity in individuals with latent TB infection (LTBI) but not in those with active TB 101,102 .HBHA, a cell surface protein, is involved in the binding of mycobacteria to epithelial cells facilitating the dissemination of infection to extrapulmonary sites and contributing to TB pathogenesis 103,104 .Studies have investigated HBHA as an antigen of diagnostic and protective value against TB [105][106][107][108] .When administered with adjuvant, immunization with purified HBHA protein resulted in an approximately 5.0-fold reduction in lung bacterial burden compared to the adjuvant control mice 28 days post-challenge 105 .Subcutaneous administration of rHBHA improved the effectiveness of BCG in the spleens and lungs of mice challenged with M. tuberculosis via the intranasal route and only the spleens of aerosol-infected mice 109 .

M. tuberculosis genomic expression library
Serological screening of M. tuberculosis genome expression libraries has been used to identify several promising T-cell antigens.Young et al. constructed a genomic expression library representing the complete M. tuberculosis genome in the λgt-11 expression vector 110 .Several studies have used this λgt-11 M. tuberculosis genomic expression library to screen TB patient sera, rabbit polyclonal sera, and murine monoclonal antibodies to identify antigenic proteins or epitopes 44,[110][111][112][113][114][115][116][117][118][119] .M. tuberculosis genomic expression library screening has been used to identify immunogenic components of ST-CF, such as the 96 amino acid antigen TB10.4 120 .The simultaneous expression of antigenic proteins TB10.4,Ag85A and Ag85B in a recombinant BCG-based vaccine expressing perfringolysin O (AFRO-1) yielded an enhanced immune response in infected mice and guinea pigs compared to perfringolysin O expressing BCG strain (AERAS-401) 121 .The expression of perfringolysin O in BCG leads to the lysis of the endosomal membrane and increased antigen presentation 122 .Mice immunized with AFRO-1 and infected with the highly pathogenic M. tuberculosis HN878 survived longer than parental BCG 1331 strain 121 .TB10.4 is a component of the vaccine constructs H4 + IC31, H1 + IC31, and Crucell Ad35 currently in the TB vaccine clinical trial pipeline (Table 1, Fig. 1).However, recent studies have proposed that M. tuberculosis uses TB10.4 as a "decoy" protein by inducing immunodominant antigen-specific CD8 T cells that poorly recognize M. tuberculosisinfected macrophages, and therefore, cannot mediate optimal protection 123,124 .Such findings highlight the critical role of choice of antigens in developing new TB vaccine candidates.
Screening of the H37Ra genomic expression library with rabbit antiserum raised against the CFP of M. tuberculosis Erdman identified two secreted serine proteases MTB32A (Rv0125) and MTB32B (Rv0983) as immunogenic antigens 125 .These antigens have been evaluated in a clinical trial with the adjuvant AS01E as a subunit vaccine M72/AS01E (Table 1, Fig. 1).Phase IIb trial studies of M72/AS01E have shown promising results.Immunization with M72/AS01E imparted ~50% protection (95% CI, 1.8 to 74.1) against active pulmonary TB disease in human immunodeficiency virus (HIV)-negative adults with latent M. tuberculosis infection 126 .Dillon et al. used pooled sera from TB patients for serological screening of the M. tuberculosis genomic expression library and identified MTB39A (Rv1196) as an immunogenic antigen.The authors showed that MTB39A elicited T-cell proliferation and IFN-γ production in PBMCs of PPD-positive healthy individuals 127 .MTB39A belongs to the PE/PPE family and is present in M. tuberculosis lysate but not in CFP, providing evidence that potent T-cell antigens are not exclusively present in CFP.Immunization of C57BL/ 6 mice with DNA vaccine encoding MTB39A reduced the lung bacillary load by 5.0-fold compared to sham-immunized mice.However, protection was lower compared to BCG-vaccinated mice (10.0-fold reduction in M. tuberculosis burden compared to sham-immunized animals) 127 .Serological screening of M. tuberculosis expression libraries has limited potential since immunogenic T-cell antigens may not necessarily produce robust antibody responses and, thus, may not be captured by this approach.In contrast, T-cell expression cloning is a technique used for the direct identification of mycobacterial antigens involved in disease control [127][128][129] .Alderson et al. developed an expression cloning approach using M. tuberculosis-specific protective CD4 + T-cell line generated from healthy PPDpositive volunteers (non-BCG-vaccinated) 129 .This study identified MTB9.9A (Rv1793), which was recognized by T-cells from healthy PPDpositive individuals but not PPD-negative volunteers 129 .Skeiky et al. generated anti-M.tuberculosis-CFP-specific CD4 + T-cell line from infected C57BL/6 mice to identify antigens linked with the early control of M. tuberculosis infection 128 .This study identified MTB41 (Rv0915c) belonging to the PE/PPE family of proteins as a candidate antigen for TB vaccine development.Mice immunized with a DNA vaccine expressing MTB41induced antigen-specific CD4 + and CD8 + responses, and this vaccination imparted protection comparable to BCG (~8.0-10.0-foldreduction in lung bacillary load of DNA vaccine immunized and BCG-immunized C57BL/6 mice compared to vector immunized mice) 128 .However, although expression cloning is a promising method, its application is limited to the identification of protein antigens.Additionally, the influence of posttranslational modifications arising from heterologous expression on the immunogenicity of antigens remains a limitation 130 .

In silico approaches
Computational vaccinology has accelerated vaccine design by enabling epitope prediction and selection of immunogenic antigens at a low cost 131 .Numerous studies have employed genome-wide screening and reverse vaccinology to identify uncharacterized immunogenic epitopes and proteins without any antigen bias.The availability of M. tuberculosis genome sequence has facilitated the identification of potential candidate vaccine antigens using comprehensive data mining and in silico tools 131,132 .Zvi et al. constructed a bioinformatics genome analysis pipeline and identified 45 top-ranking candidate antigens covering various stages of TB disease 133 .Among the antigens discovered by Zvi et al., at least 17 candidate antigens have been evaluated in clinical trials and preclinical models.The majority of the 45 top-ranked antigens included DosR-regulated proteins, reactivation/ resuscitation proteins, cell wall proteins, classical vaccine candidates, and conserved hypothetical proteins.Based on the list of 45 top-ranked antigens, rBCG AERAS-407 was designed to overexpress (i) classical antigens such as Ag85A and Ag85B, (ii) dormancy or resuscitation antigens including RpfA, RpfC, and RpfD, and (iii) M. tuberculosis specific antigen Rv3407.Compared to BCG-immunized mice, immunization with rBCG AERAS-407 resulted in a more pronounced antibody and cellular immune response.rBCG AERAS-407 immunized mice showed a greater reduction in viable bacilli count in lungs and spleens compared to BCG vaccinated mice against M. tuberculosis Erdman at ten weeks post-challenge 134 .
In a genome-wide approach, five complete M. tuberculosis genomes and 16 draft assemblies were screened in silico for 15-mer peptides predicted to bind to the most frequently expressed human leukocyte antigen (HLA) class II alleles 135 .The peptides were subsequently analyzed for ex vivo IFN-γ production by PBMCs from LTBI donors.A total of 82 antigens were identified that are frequently recognized by LTBI donors.Among these, 34 antigens have not been previously classified as T-cell antigens 135 .Furthermore, several of these antigens were uniformly recognized by LTBI donors from different geographical locations, indicating their potential use in developing vaccines targeting diverse populations 136 .Using in silico epitope prediction tools, De Groot et al. identified class II MHC binding epitopes from known secreted antigens such as MPT64, Ag85B, MPT70, MPT63, 38 kDa protein, 14 kDa, 16 kDa, 19 kDa, and 32 kDa protein as well as from ORFs containing signal sequences 137 .TB 001, a DNA vaccine construct overexpressing 24 selected epitopes, stimulated epitope-specific T-cell responses in HLA DRB1*0101 transgenic mice expressing the human MHC haplotype of class II antigens 137 .IKEPLUS is a genetically modified M. smegmatis strain expressing ESX-3 type VII secretion system of M.
tuberculosis, and immunization of mice with IKEPLUS resulted in strong bactericidal CD4 + T-cell responses and prolonged survival of C57BL/6 mice after M. tuberculosis challenge 138 .A synthetic peptide library of 880 nonoverlapping peptides was constructed by in silico prediction of possible 15mer peptides from the genome sequences of M. smegmatis and M. tuberculosis to examine the range of CD4 + T-cell epitopes in IKEPLUSimmunized mice.Screening of the peptide library by an IFN-γ ELISPOT assay using CD4 + T-cell isolated at two weeks post-immunization from spleens of IKEPLUS immunized C57BL/6 mice showed that mycobacterial ribosome was a major target of CD4 + T-cell 139 .The study identified a specific immunogenic peptide within the mycobacterial ribosomal large subunit protein RplJ (Rv0651).Compared to BCG-immunized mice, CD4 + T-cells from IKEPLUS-immunized mice responded significantly to the 15-mer RplJTB 146 -160 epitope.In another study, boosting of BCG primed immunity with the M. tuberculosis RplJ protein resulted in significantly reduced lung pathology and bacterial burden in mediastinal lymph nodes compared to naïve or BCG-immunized mice 140 .These findings suggest that ribosomal proteins are potential sources of antigenic targets that are recognized by protective CD4 + T-cells.
In addition to CD4 + T-cells, HLA class I-restricted CD8 + T-cells play an essential role in controlling M. tuberculosis infection and mediating optimal host response in small animal models, non-human primates, and humans 141 .However, conventional antigen discovery approaches have only identified a few CD8 + T-cell epitopes.The repertoire of CD8 + T-cell epitopes identified in M. tuberculosis has increased as a result of in silico epitope prediction approaches.In one of the first genome-wide screens to identify CD8 + T-cell epitopes, all 3924 M. tuberculosis ORFs were searched for HLA-B*35 restricted CD8 + T-cell epitopes, common HLA-B type in West Africa 142 .Among the 479 predicted CD8 + T-cell epitopes, 13 most promising epitopes belonged to uncharacterized mycobacterial antigens and elicited a CD8 + T-cell response in healthy BCG-vaccinated donors naturally exposed to mycobacteria 142 .Using genome-wide in silico epitope prediction tools and immunological screening, Tang et al. identified CD8 + T-cell epitopes presented by HLA class I supertypes that account for 80% of the human population 143 .The antigens that were either secretory or being evaluated in various TB vaccine candidates had the highest frequency of predicted CD8 + T-cell epitopes.Following immunological screening, a number of new M. tuberculosis peptides likely to be presented by CD8 + MHC class-I were identified in healthy PPD-positive donors.In individuals with cured TB disease, a set of epitopes was shown to trigger mono-, dual-, or triple-cytokine-producing CD8 + T-cell responses, indicating the polyfunctional nature of these M. tuberculosis peptide-specific CD8 + T-cells 143 .Similar epitope prediction in silico tools have been used to identify promiscuous epitopes in dormancy antigens 144 .Promiscuous epitopes are expected to have a broader population coverage since they are predicted to bind ten or more HLA alleles.Several of the promiscuous epitopes are conserved among different M. tuberculosis strains and have a larger population coverage than ESAT-6 and CFP-10 144 .In a T-cell-focused genomewide search of immunodominant CD8 + T-cell epitopes, Lewinsohn et al. identified a set of 389 unique M. tuberculosis proteins likely to possess CD8 + T-cell epitopes 145 .Further, a 15-mer synthetic peptide library was synthesized, and this peptide library (representing 10% of the M. tuberculosis proteome) was screened with CD8 + T-cells isolated from LTBI or active TB donors.This study identified three CD8 + T-cell antigens, EsxJ (Rv1038c), PE9 (Rv1088), and PE_PGRS42 (Rv2487c) as promising vaccine antigens 145 .
Mycobacterial antigens are presented by classical as well as nonclassical HLA molecules.Unconventional HLA molecules such as HLAclass 1b exhibit limited polymorphism.HLA class 1b molecules such as HLA-E present M. tuberculosis epitopes triggering CD8 + effector T-cell activation.HLA-E expression is not affected by HIV co-infection, which is highly prevalent in TB-endemic countries 146,147 .In the first genome-wide effort to identify CD8 + T-cell peptide epitopes presented by human HLA-E, in silico tools were used to scan the M. tuberculosis genome for putative HLA-E binding peptide epitopes 148 .This study identified 69 potential HLA-E epitopes and most of the peptides displayed CD8 + T-cell proliferation in PBMCs derived from PPD-positive donors and BCG immunized infants 148 .These CD8 + T-cell recognizing peptides presented by HLA-E represented a new population that exerts cytolytic functions, activates B-cells, and produces Th2 cytokines instead of Th1 cytokines 149,150 .

Other genome-wide approaches
Unbiased antigen discovery approaches such as genome-wide gene expression studies have identified genes that are differentially expressed during in vivo infection with M. tuberculosis 151 .A set of in vivo-expressed M. tuberculosis genes (IVE-TB) genes was selected based on their real-time in vivo expression in the lungs of genetically related mice strains representing a spectrum of susceptibility to M. tuberculosis infection 151 .Several IVE-TB genes, such as Rv1363c, Rv1956, Rv2034, Rv2324, Rv2380c, Rv3353c, and Rv3420c, were identified to be highly immunogenic as measured by antigen-specific IFN-γ production by mice splenocytes.These IVE-TB antigens were also recognized by immune cells from TST-positive individuals but not recognized by cells from healthy controls 151 .IVE-TB genes also induced strong multifunctional T-cell responses in PBMCs isolated from LTBI donors 151 .Rv2034, implicated in stress response and regulation of lipid metabolism, has been evaluated as a vaccine candidate in mice and guinea pigs 152 .Rv2034 protein was highly immunogenic in HLA-DR transgenic mice and elicited cellular and humoral immune responses.Rv2034 when administered as a fusion protein along with Ag85B and ESAT-6 (H1-Rv2034/CAF09) showed a significant reduction of bacillary load in the lungs and spleen of M. tuberculosis infected guinea pigs by 13.0and 436.0-fold, respectively, relative to the unvaccinated group.The levels of protection were comparable to those observed in BCG-vaccinated guinea pigs 152 .A subsequent study analyzed the expression of 2068 M. tuberculosis genes to identify highly upregulated IVE-TB genes during the early and late stages of infection 153 .In this study, gene expression data from the lungs of resistant and susceptible mice infected with M. tuberculosis identified 194 genes with altered expression.The most promising genes were further shortlisted using the following criteria: (i) top 15% genes expressed during the last six weeks of M. tuberculosis infection, (ii) hyper conservation and the presence of HLA class Ia and II binding epitopes, and (iii) the presence of homologs in M. leprae 153 .A total of 48 out of these 194 differentially expressed IVE-TB genes were recombinantly expressed and evaluated for immunogenicity using PBMCs from double responders (ESAT-6/CFP-10 positive and PPD-positive), single responders (ESAT-6/CFP-10 positive or PPD-positive) and non-responders (ESAT-6/CFP-10 negative and PPDnegative) to M. tuberculosis.Among these, 29 IVE-TB proteins triggered a cytokine response from PBMCs from M. tuberculosis exposed individuals, and nine of these antigens (Rv3615, Rv2029, Rv3353, Rv1733, Rv0826, Rv2215, Rv1791, Rv2873, and Rv2626c) triggered the secretion of cytokine response (IP-10, TNF-α, and IL-17) without induction of IFN-γ response 153 .Another study was performed in three different mice strains, including susceptible (C3HeB/FeJ) and resistant (C57BL/6J and Balb/c) murine strains, to identify IVE-TB-antigens that are recognized by T-cells following M. tuberculosis infection or BCG immunization 154 .This study identified 11 IVE-TB-antigens that were recognized in both TB-resistant and susceptible mice strains.These antigens induced IFN-γ, TNF-α, and IL-17 across different organs in resistant and susceptible mice.In addition, three IVE-TB antigens (Rv0470, Rv1733, and Rv3616) were strongly recognized by M. tuberculosis infected as well as BCG-immunized C3HeB/FeJ mice and induced strong IFN-γ response, suggesting their potential for use in primeboost approach 154 .
Genome-wide antigen discovery efforts have also increased the repertoire of CD8 immunodominant antigens.IFN-γ ELISPOT assay-based screening of synthetic peptide library against human CD8 + T cells identified several immunodominant CD8 T-cell antigens.Among these, PPE15 (Rv1039c), PPE51 (Rv3136), PE12 (Rv1172c), and PE3 (Rv0159c) have been evaluated as potential candidate antigens for developing TB subunit vaccines [155][156][157] .In addition to being immunodominant CD8 T-cell antigens, these antigens also induced strong CD4 + T-cell responses in TB patients and individuals with LTBI.In subsequent studies, these antigens have been incorporated in a replication-deficient chimpanzee adenovirus (ChAdOx1), and protective efficacy was analyzed following intranasal vaccination in murine M. tuberculosis challenge experiments 157 .ChAdOx1.PPE15 and ChAdOx1.PPE51 reduced the lung bacillary load compared to the naïve control group (by approximately 10.0-fold), but the protection afforded was less compared to the BCG-immunized group.However, the lungs and splenic bacillary load in the BCG-immunized group was further decreased by 3.0-fold and 5.0-fold, respectively, upon booster with ChAdOx1.PPE15.

Recent developments in antigen discovery
Recent advancements in single cell T-cell receptor sequencing technologies (scTCR-seq) and improved in silico analytical tools such as GLIPH2 (algorithm to cluster TCRs that recognize the same epitope) have allowed unprecedented throughput and efficiency in the profiling of TCR specificities 158,159 .Leveraging on two well-characterized cohorts of M. tuberculosis-infected individuals, some of whom remained well (controllers) and others who progressed to active TB disease (progressors), Musvosvi et al. used scTCR-seq and the GLIPH2 algorithm to sequence M. tuberculosis-reactive T-cells (mostly CD4) and identify putative protective (enriched in controllers) or non-protective (enriched in progressors) TCR similarity groups 159 .Further, genome-wide antigen screening analysis allowed the identification of epitopes from two M. tuberculosis proteins, PE13 (Rv1195c) and CFP-10.These antigens were recognized by the TCR similarity group associated with controllers as putative vaccine targets.Interestingly, the majority of TCR similarity clusters were identified with equal frequencies in controllers and progressors 159 .This implies that M. tuberculosis expresses a number of antigens, which, despite inducing T-cells, have little impact on the outcome of TB infection.In addition, the authors showed that EspA (Rv3616c) is only recognized by T-cell clones in progressors 159 .Previously, it has been shown that EspA and CFP-10 are mutually dependent on each other for secretion in M. tuberculosis 39 .The opposite effect observed for TCR recognition of CFP10 and EspA, as well as the neutral effect of certain T-cell inducing antigens on outcome of TB infection, highlights the inadequacies of empirically selecting antigens and the need for systematic screening of T-cell specificities and TB outcome.
Immunopeptidomics is a recent advancement in mass-spectrometrybased approach for the precise identification of MHC-bound peptide epitopes 160 .This methodology identified 94 and 43 mycobacterial peptides presented by MHC class-II and MHC-I, respectively, in BCG-infected macrophages 161 .These peptides belonged to antigens predominantly expressed in infected macrophages, and a majority of them were membrane-associated and involved in lipid biosynthesis.Among these, three new antigens, GlfT2 (galactofuranosyl transferase, BCG_3870c (Rv3808c)), IniB (isoniazid inducible protein, Rv0341), and Fas (fatty acid synthetase, Rv2524c) were expressed in viral vectors and evaluated as boosters for BCG immunization 161 .A combination of these three antigens, when administered in a prime-boost regimen in intradermal BCGimmunized mice, showed significantly lower CFU counts in the lungs and spleen post-M.tuberculosis challenge compared to BCG-immunized mice.Immunopeptidomics has also been used to identify ligands bound to non-classical highly conserved HLA-E molecules from M. tuberculosisinfected human cells 162 .In this study, 28 HLA-E ligands were identified from 13 M. tuberculosis proteins.Among these, a peptide epitope was strongly and broadly recognized by CD8 + T-cells from donors with M. tuberculosis infection, latent infection, and healthy controls, suggesting it may have scope for vaccine strategy targeting donor-unrestricted T-cells.
Traditionally, TB vaccine antigen discovery approaches have focused on the identification of immunogenic protein antigens.However, mycobacterial lipid antigens are also attractive candidates for subunit TB vaccine development.Studies have shown that immunization of guinea pigs with formulations of mycobacterial lipid antigens imparts modest protection against M. tuberculosis challenge compared to unvaccinated guinea pigs 163 .Using a combination of protein and lipid antigens in multi-component subunit vaccines might hold promise for future TB vaccines.In a similar approach, immunization of humanized mice with a dual encapsulation of Ag85B and lipid antigen mycolic acid (MA) resulted in the activation of both protein and lipid antigen-specific T-cells 164 .Mycobacterial outer membrane vesicles (OMVs) containing membrane and cell wall components can be a source of mixed protein and lipid antigens and stimulate mixed humoral and cellular immune responses.OMVs have been evaluated in animal models, demonstrating their ability to impart protection against M. tuberculosis challenge [165][166][167] .However, heterogeneous size and low stability limit the use of OMVs as vaccines.OMV-coated nanoparticles have been shown to have improved stability and enhanced immuno-stimulatory properties 168 .
The majority of vaccine candidates proposed for TB vaccine development are focused on T-cell-mediated immunity.Earlier studies using M. tuberculosis infected cynomolgus macaques showed the presence of B-cell clusters surrounding granuloma that secrete M. tuberculosis-specific antibodies 169 .Whilst a functional role for humoral immunity in protection against M. tuberculosis remains poorly defined, a number of cohort studies have provided supporting evidence of the role of antibodies in the control of M. tuberculosis infection by mucosal immunity [170][171][172][173][174][175][176][177][178][179] .Studies have also demonstrated the role of monoclonal antibodies in imparting protection against TB in murine models 180 .Antibodies isolated from M. tuberculosisexposed healthcare workers could confer moderate (~2.0-to 3.0-fold) protection against M. tuberculosis infection in aerosol mouse challenge model 173 .In addition, monoclonal antibodies against mycobacterial phosphate transporter subunit PstS1 (Rv0934) isolated during active TB infection showed inhibitory activity against intracellular growth of BCG and M. tuberculosis in THP-1 macrophages 9 .These observations suggest that protective antibody responses can be generated during active TB disease.Recently, a thorough analysis of IgG/IgA memory B-cell repertoire from an occupationally exposed, asymptomatic healthcare worker donor identified a monoclonal antibody targeting the cell membrane-associated virulence factor LpqH (Rv3763) as a promising candidate for future vaccine strategies 10 .Anti-LpqH human monoclonal antibody showed a reduction of M. tuberculosis counts in murine lungs when compared with a non-specific human isotype control 10 .These antibodies also displayed significant M. tuberculosis growth restriction in an ex vivo human whole blood mycobacterial growth inhibition assay 10 .Existing data strongly suggests that protective antibodies are directed against surface antigens of M. tuberculosis.Intravenous administration of antibody directed against surface antigen lipoarabinomannan (LAM) in intravenous M. tuberculosis-infected mice significantly reduces the bacterial burden in the lungs and spleens of infected animals and prolongs their survival 7 .Taken together, unbiased screening for potentially protective antibodies holds promise in identifying new targets for future TB vaccine development.Developing vaccines that target both cellular and humoral immunity may be an effective vaccination strategy against TB.However, there is a lack of data on epitopes that likely stimulate functional antibodies against M. tuberculosis, and cloning and characterization of protective human monoclonal antibodies can be a promising tool to identify antigens that induce such antibodies upon vaccination.
There has also been an increase in multi-epitope-based peptide vaccines for TB (TB MEVs) due to the recent advances in immunoinformatics and computational immunology.Immunoinformatics has expedited vaccine design by enabling in silico assessment of immunological properties of MEVs, including their ability to induce the proliferation of innate and adaptive immune cells and capacity to stimulate cytokine release from immune cells 181 .MEVs have demonstrated several advantages, such as appropriate safety profile, lower risk of adverse effects, and low manufacturing costs 182 .Helper T-lymphocyte (HTL) epitopes, cytotoxic T-lymphocyte (CTL) epitopes, and B-cell epitopes derived from several antigenic proteins of M. tuberculosis have been combined to create several TB MEVs.Multi-epitope vaccines that combine T-cell and B-cell epitopes can induce a broad range of immune responses.MEVs have been designed using epitopes from experimentally confirmed known antigenic proteins and antigens [183][184][185][186][187] .In silico tools are used to screen epitopes for their antigenicity, allergenicity and toxicity.These epitopes are fused with appropriate linkers and combined with TLR agonists as adjuvants.Subunit vaccine preparations of these MEVs can be purified by recombinant expression in E. coli 188 .The structural, physiochemical, and immunological attributes of the MEVs are validated using immunoinformatic tools, but most of these vaccines still need to be evaluated in vitro and in vivo for their immune and efficacy studies.∼50 mRNA-based vaccines against SARS-CoV-2 were approved by the FDA in just three years.mRNA vaccines have the potential for quick and scalable vaccine production.Furthermore, mRNA vaccines address the safety concerns associated with the use of DNA vaccines that may cause insertional mutagenesis.There has been limited usage of RNAbased vaccination platforms in TB vaccine development.A recent study used immunoinformatics to design a multi-epitope mRNA vaccine based on predicted immunogenic epitopes from nine M. tuberculosis proteins.These proteins have been shown to modulate host immune responses by inducing epigenetic modifications 189 .BioNTech (Mainz, Germany) recently announced plans to test multi-antigenic mRNA-based TB vaccines BNT164a1 and BNT164b1 (ClinicalTrials.govIdentifier: NCT05547464) in phase I clinical trials for their safety and immunogenicity.Larsen et al. designed and evaluated a novel Venezuelan equine encephalitis (VEE) virus-based replicating RNA (repRNA) vaccine platform against M. tuberculosis infection in mice 190 .The ID91 repRNA is a multi-antigenic vaccine that harbors four M. tuberculosis antigens, EsxV (Rv3619, ESAT6like protein), RpfD, PPE60 (Rv3478), and Ag85B in a VEE repRNA backbone.The efficacy of ID91 repRNA was compared to ID91, which was generated as a fusion protein formulated with a synthetic TLR4 agonist as a prophylactic vaccine against M. tuberculosis challenge in C57BL/6 mice.Immunization of mice with ID91 repRNA generated T-cell responses to a broader range of epitopes compared to ID91 protein subunit vaccine.Despite the better immune response, ID91 repRNA moderately reduced the lung bacterial burden compared to sham-immunized animals.In comparison, the heterologous RNA-prime protein-boost strategy was more effective in reducing the lung bacterial load 190 .
Taken together, advancements in antigen discovery approaches for vaccine candidates have exponentially increased the flux of antigens identified for designing vaccination strategies against M. tuberculosis.However, the majority of candidate antigens under clinical development were discovered decades ago.Small animal models are important preclinical models (i) for the evaluation of immunogenicity and protective efficacy of vaccine candidates and (ii) for prioritization of candidates into clinical trials.Although indispensable, no animal model fully recapitulates human TB disease.In the next section, we discuss recent advancements in preclinical evaluation models for TB vaccines.

TB vaccine preclinical evaluation models
Designing TB vaccine candidates with increased efficacy is facilitated by the advancements in antigen discovery approaches.Despite these advances, there are numerous challenges in developing a vaccine that is safe and imparts better protection than BCG in humans.These include (i) determining the entire antigenome of M. tuberculosis, (ii) choosing antigens or combinations that elicit optimal immunogenic responses, (iii) selecting antigens for epitope prediction, (iv) the necessity of combining T-cell and B-cell epitopes, (v) identifying appropriate vaccine correlates of protection and (vi) identifying preclinical evaluation models with predictive value for vaccine efficacy in humans.Animal models are valuable tools for studying immunogenicity, safety, and vaccine efficacy.Preclinical testing in animal models allows the screening of hundreds of vaccine candidates and helps prioritize a few for evaluation in human clinical trials.Various in vitro models and animal models, including mice, guinea pigs, rabbits, non-human primates and cattle have been used to study the efficacy of TB vaccines (Fig. 2).The advantages and disadvantages of conventional animal models for TB research have been extensively reviewed previously [191][192][193] .These animal models show considerable differences in their susceptibility to infection, the extent of organ involvement, and the type of immune response.For instance, immunization with MVA85A, a viral vectored vaccine constructed using modified vaccinia Ankara virus expressing antigen 85A, induced potent Th1 responses and imparted better protection than BCG in preclinical animal models but failed to deliver superior protection compared to BCG in Phase IIb clinical trial [194][195][196] .In addition, the route of vaccine administration also significantly impacts the outcome of the infection.For example, BCG is administered intradermally to the human population and shows variable efficacy (0-80%) against pulmonary TB 2,197 .A recent study showed that intravenous administration of BCG in non-human primates (Macaca mulatta) generated significantly enhanced CD4 + and CD8 + T-cell responses in blood, spleen, bronchoalveolar lavage, and lymph nodes compared to intradermal or aerosol administration of the vaccine.Further, intravenous immunization of BCG imparted protection in 90% of the macaques post M. tuberculosis challenge.Among these, 60% of the animals showed no detectable sign of infection 13 .The authors showed that intravenous immunization of non-human primates with BCG reduced the lung bacterial burden by greater than 1,00,000-fold compared to intradermal BCG immunization 13 .A higher proportion of mycobacteria-specific T-cells in airways, tissue-resident memory T-cells, and robust immunoglobulin IgM responses were identified as correlates of protection in macaques immunized intravenously with BCG 13,198 .Intravenous BCG immunization also protected simian immunodeficiency virus (SIV)-infected macaques from M. tuberculosis infection 199 .These studies demonstrate that BCG administration via the intravenous route is more immunogenic and efficacious than the conventional intradermally administered BCG.These observations suggest that intravenous administration of vaccines might boost the protective efficacy of vaccines that are under clinical development.
To eliminate TB, newer preclinical TB vaccine evaluation models are required to address key limitations of the existing animal models, which do not fully recapitulate features of human disease.In this review article, we discuss new in vitro, animal, and human challenge models that have been developed to complement the ongoing efforts in TB vaccine development and evaluation.These newer models may offer valuable insights into the quest to completely eradicate TB.

Human challenge model
Human challenge (HC) models have been routinely used for various diseases such as malaria, influenza, dengue fever, and typhoid [200][201][202][203] .The advantages of the HC model include (i) a significant reduction in time and costs associated with preclinical assessment in animal models and (ii) identification of the correlates of protection.BCG is currently the strain of choice for infection in the HC model.BCG is safe for use in humans and has a 99.95% sequence similarity to M. bovis 204 .BCG can self-replicate and cause limited infection in immunocompetent animals and humans.Multiple approaches have been used to develop a less virulent self-replicating strain of M. tuberculosis.One strategy involves using M. tuberculosis auxotrophic strains that are attenuated for growth in macrophages and in vivo 205 .Another alternative approach entails using genetically modified strains expressing essential enzymes in the presence of either unnatural amino acidbased regulatory systems, regulated promoters, or regulatable self-kill switches.Self-kill switches are regulatory circuits that, in response to specific stimuli, can maintain essential gene expression or block the expression of toxins 206 .Without exogenous signals, these regulatory circuits block the expression of genes of interest or induce the expression of toxins [206][207][208] .
Angela et al. developed the first HC model, where intradermal BCG challenge was used as a surrogate for M. tuberculosis infection 209 .The rationale for choosing BCG as the infecting strain was based on the assumption that an effective vaccine candidate against M. tuberculosis will also reduce the BCG burden 209 .In this study, naive or BCG-vaccinated healthy volunteers were intradermally challenged with BCG, and the load of BCG was determined from skin biopsy specimens (challenge site) using culture and polymerase chain reaction (PCR).The authors showed that BCG persisted in the skin for more than four weeks and displayed a spectrum of protection among BCG-immunized groups.The majority of the cells in the blister fluid were neutrophils.PCR analysis of skin biopsy samples from vaccinated individuals showed a 10.0-fold reduction in challenge BCG counts compared to naïve individuals [210][211][212] .However, the culture data showed no difference in challenge load between the naive and BCG-vaccinated groups 209 .Earlier studies have shown that booster of BCGimmunized animals with MVA85A resulted in better protection in animals 213,214 .Harris et al. used BCG as the human challenge strain to evaluate the efficacy of MVA85A 215 .Unvaccinated and BCG-vaccinated individuals were immunized with MVA85A followed by intradermal challenge with BCG.It was observed that BCG challenge was well tolerated by individuals with an expected localized inflammatory reaction.The authors observed that prior BCG vaccination imparts some protection against subsequent BCG challenge.Further, a significant reduction in challenge strain was also detected in the BCG-MVA85A immunized group compared to the naive group.However, boosting BCG-vaccinated individuals with MVA85A resulted in no further reduction in the counts of challenge strain.Taken together, the results obtained are consistent with the outcomes of MVA85A phase 2b trials in BCG-vaccinated infants 6,215 .The authors showed that vaccination with BCG-MVA85A for seven days resulted in significantly higher ex vivo IFN-γ responses to Ag85A compared to BCG-naïve MVA85A vaccinated individuals, and this was consistent with previous reports in humans 215,216 .A lung-oriented controlled human infection model (CHIM) has been evaluated in South African volunteers using live BCG or sterile PPD bronchoscopically administered into the lungs establishing feasibility and safety of this human infection model with only mild adverse effects 217 .Recently, Wang et al. have described the construction of safe and effective M. tuberculosis strains for human challenge studies.This newly developed M. tuberculosis strain called the triple-kill-switch (TKS) strain has three kill switches tightly regulated via tetracyclines and trimethoprim.This TKS strain cannot replicate and grow in axenic cultures and mice in the absence of tetracyclines and trimethoprim.In contrast, under permissive conditions, the TKS strain displayed immunogenicity and antibiotic susceptibility similar to the parental strain 218 .These studies suggest that BCG and engineered M. tuberculosis strains might be considered for the human challenge model and provide a valuable tool for assessing vaccine-induced protection prior to clinical evaluation.

Human PBMC-based in vitro models and organotypic cell culture models
In vitro three-dimensional cultures have also emerged as useful tools for studying tissue development, organogenesis, and stem cell behavior 219 .The hallmark of TB disease manifestation is granuloma formation 220 .To develop a three-dimensional culture model, PBMCs from healthy donors were incubated with partially purified PPD protein coated on cyanogen bromideactivated sepharose beads.In this model, the authors observed sequential recruitment of monocytes and lymphocytes around PPD-coated beads 221 .The cellular structure, thus formed, resembled the naturally occurring granulomas.The detailed analysis of these structures revealed that activated macrophages with large vesicles were the most prominent cell type.In addition, a few multinucleated giant macrophages and other non-immune cells, like epithelioid cells, were also recruited to the beads.Aliabbas et al. used PBMCs to identify and evaluate the T-cell activation by secretory antigens extracted from M. tuberculosis cultures at different stages of growth.PBMCs stimulated with CFPs isolated from the late logarithmic growth phase of M. tuberculosis culture exhibited better T-cell activity (measured by secretion of IFN-γ, TNF-α, and IL-12) when compared to the PBMCs induced with CFPs prepared from BCG strain 222 .Kapoor et al. developed an in vitro model of human TB granuloma using human primary leukocytes, where M. tuberculosis enters a dormancy-like state.In this model, PBMCs from healthy donors were incubated over a collagen-coated matrix in the presence of a low dose of M. tuberculosis for eight days, forming microscopic granulomas 223 .M. tuberculosis residing in these in vitro micro-granulomas exhibited characteristics of dormant mycobacteria, including (i) loss of acid-fastness, (ii) accumulation of lipid bodies, (iii) rifampicin resistance, and (iv) changes in gene expression.The salient features of this in vitro granuloma model included (i) lymphocyte aggregation around infected macrophages, (ii) formation of multinucleated giant cells, (iii) secretion of inflammatory cytokines by host cells, (iv) features of M. tuberculosis dormancy, and (v) reactivation of M. tuberculosis upon immune suppression by anti-TNFα monoclonal antibody treatment.In addition to human PBMC-based in vitro models, researchers have also generated an in vitro human lung tissue model using lung-tissue-specific epithelial cell lines, fibroblasts, primary human monocytes, and macrophages 224,225 .This organotypic culture model showed cellular characteristics of lung epithelium, such as even distribution of macrophages throughout the epithelium, extracellular matrix production, stratification of epithelium, and mucus secretion at air-liquid interphase 225,226 .Introduction of virulent M. tuberculosis H 37 Rv infected macrophages in this model resulted in granuloma-like clustering of macrophages at the site of infection resembling early events involved in TB granuloma formation 225 .Further, the authors also demonstrated that RD1 region and ESAT-6 secretion are necessary for the early-granuloma formation in this experimental lung tissue model reminiscent of in vivo studies 225 .This methodology has opened avenues to understanding host-pathogen interactions.Incorporating immune cells and vasculature to mimic the dynamic microenvironment of the lung airways can further improve the organoid models and enable us to study early immune responses post-infection.

Genetically diverse mouse models
Host genetic variation is an important determinant of infection outcome.The genetic factors that govern the outcome of M. tuberculosis infection in humans are largely unknown 227 .Studies have shown that certain inbred mouse strains commonly used for vaccine efficacy testing are genetically more susceptible to M. tuberculosis infection compared to others.Based on the mean survival time (MST) upon infection with M. tuberculosis, mice strains have been categorized into two distinct groups.BALB/c, C57BL/6, and C57BL/10 are highly resistant to infection with MST of greater than 250 days.In comparison, DBA/2, C3H/HeJ, CBA, and 129/SvJ are highly susceptible to infection with MST of less than 114 days 228 .The genetic cross between C57BL/6 J (resistant) and C3HeB/FeJ (susceptible) was used to identify the sst1 locus (susceptibility to tuberculosis locus 1) that regulates M. tuberculosis multiplication in lung tissues and disease progression 229 .The sst1 locus encodes for an intracellular pathogen resistance 1 (ipr1) gene, which regulates innate immunity to M. tuberculosis infection.C3HeB/FeJ mice harbor a unique mutation in the ipr1 allele, which leads to loss of expression of the ipr1 gene, whereas other C3H strains like C3H/HeJ, C3H/ HeSnJ, and C3H/HeOuJ encode for the wild-type protein 230 .Studies have shown that a mutated ipr1 allele results in a defect in macrophage-mediated innate immune response upon infection with either M. tuberculosis or Listeria monocytogenes 230 .Ipr1 controls the multiplication of M. tuberculosis and L. monocytogenes by regulating apoptosis of infected cells.The lack of expression of Ipr1 results in necrosis and escape of M. tuberculosis from the phagolysosome 230 .
The resistance of mice to BCG is linked to the antimicrobial resistance gene nramp1 (natural resistance-associated macrophage protein) 231 .nramp1 encodes for an integral membrane transport protein, and the susceptibility allele harbors a non-conservative glycine-to-aspartic-acid substitution within the predicted transmembrane domain 232 .To determine whether nramp1 contributes to resistance to M. tuberculosis infection, nramp1 susc/susc and nramp1 res/res mice were infected with M. tuberculosis via intravenous or aerosol route.Both mice strains were susceptible to infection with M. tuberculosis at comparable levels 231,233 .These observations demonstrated that nramp1 is not involved in resistance to M. tuberculosis infection.Inbred mice have been widely used for TB research, but a significant limitation of this model is that they fail to mimic the genetic heterogeneity seen in humans 234,235 .Optimally utilizing the existing genetic diversity within the mouse species can enhance its relevance and mirror essential pathological features resembling those in humans.The diversity outbred (DO) model is derived from partially inbred mice strains and is maintained by randomized crossing to create a diverse experimental population that reduces erroneous interpretations due to strain-specific effects 236 .DO mice have been used as a tool to study (i) susceptibility to M. tuberculosis infection, (ii) variability in inflammatory responses in a heterogeneous population, and (iii) identify immune correlates of TB disease 237,238 .Ahmed et al. performed a detailed analysis of the global blood transcriptomics data obtained from the lungs of DO mice and primates and found that the expression of 13 genes (hist2h2bb, pram1, icam1, kctd12, nub1, h2afj, folr2, h3f3a, dnttip1, ltbr, mrpl23, mfsd14b, and scarf1) https://doi.org/10.1038/s41541-024-00834-yReview article npj Vaccines | (2024) 9:57 correlated well with active disease progression across species 239 .Among these, Scarf1 plays a crucial role in regulating innate immunity, immunopathogenesis, and tissue destruction, and is involved in apoptosis.In agreement, scarf1 knockout mice showed reduced disease compared to wild-type mice following M. tuberculosis infection 239 .The expression of sept4 encoding a nucleotide-binding protein involved in cytoskeleton rearrangements was increased in humans and macaques following infection with M. tuberculosis.However, in mice tissues, sept4 expression remained unchanged during infection 239 .Therefore, these observations suggest Sept4 might function differentially in mice when compared to primates and humans.Serping1 (serpin family G member 1), a highly glycosylated plasma protein, negatively regulates the complement system.Despite increased expression upon infection with M. tuberculosis, the extent of disease progression was similar in wild-type and serping1 −/− mice at different stages of infection 239 .Tap1 is important for antigen presentation to CD8 + T-cells and was upregulated upon infection with M. tuberculosis.In agreement, it was reported that tap -/-mice are unable to control bacterial burden during acute and chronic stages of disease 239 .
DO mice are well suited for studying vaccine efficacy due to their genotypic and phenotypic heterogeneity.DO mice challenged with low doses of M. tuberculosis display a range of disease susceptibility and progression.Further, immunization of DO mice with BCG is able to impart protection, resulting in increased survival and reduction in organ bacterial load 240 .A recent study showed that intravenous immunization of DO mice with BCG imparted moderately better protection compared to intradermally immunized DO mice 241 .In addition to the DO model, the Collaborative Cross (CC) mouse model has also emerged as a useful model to understand the impact of host genetic background on protection imparted by vaccination 242,243 .The CC mouse model was developed using eight diverse founder strains and recapitulated the heterogeneity of the human genome [244][245][246][247] .The CC mouse panels have been used to evaluate the protection imparted by subcutaneous immunization of BCG against M. tuberculosis challenge 242 .To investigate the impact of the host genetic background on the protective efficacy of BCG against M. tuberculosis challenge, 24 distinct CC strains were subcutaneously vaccinated with BCG.The authors showed that immunization with BCG protects half (13 out of 24 CC strains) of the mouse strains, suggesting a high degree of variability among the CC mouse strains.These observations suggest that host genetics has a major influence on BCG-induced immunity against M. tuberculosis infection 242 .Therefore, identifying the factors that regulate immunity will improve our understanding of host-pathogen interactions and help design more effective TB vaccines.
Another factor that limits the use of traditional mouse models of TB disease is the challenge dose of M. tuberculosis.The infection dose in "conventional" mice models typically varies between ~50-100 bacterial colony-forming units (CFUs), resulting in uniformly high lung bacterial burdens, which are associated with progressive inflammatory disease and poorly organized granulomas 248 .On the contrary, only ~1-3 bacilli are sufficient to establish disease in humans in most cases 249,250 .To mimic human-like infection conditions, Plumlee et al. developed an ultra-low dose (ULD) mice infection model 248 .The authors showed that mice infected with ~1-3 CFUs of M. tuberculosis exhibited highly heterogeneous bacterial burdens and wellcircumscribed granulomas that shared features with human granulomas.This study also demonstrated that transcriptional signatures derived from whole-blood of ULD or conventional dose mice model correlated well with lung bacterial burden and predicted M. tuberculosis infection outcomes across species, including the risk of progression to active TB in humans 248 .In another study, the ULD mouse model was used to assess the protective efficacy of the attenuated ΔlprG mutant strain of M. tuberculosis.The authors showed that immunization of mice with ΔlprG strain imparted significantly better protection against aerosol M. tuberculosis challenge than immunization with BCG 251 .Immunization of mice with ΔlprG reduced lung bacillary load by ∼20.0-fold and 5.0-fold, respectively, compared to the naive and BCG-immunized group 251 .

Humanized mice model
Humanized mice are immune-compromised mice engrafted with human cells or tissues.These mice are deficient in mature T and B lymphocytes and harbor a mutation in the prkdc locus, which is involved in the rearrangement of T-cell Receptor and Immunoglobulin genes 252 .The major disadvantage of the humanized mice model is graft rejection.The major advantage associated with humanized mice is that these mice mimic the essential characteristics of the human immune response to M. tuberculosis infection.Rottenberg et al. showed that infection of humanized mice with M. tuberculosis resulted in human-like granulomas 253 .Subsequently, this model has been used to study immune responses and evaluate the efficacy of various drug regimens against M. tuberculosis.Calderon et al. generated humanized bone marrow-liver-thymus (BLT) mice by transplanting human fetal liver and thymus supplemented with CD34 + fetal liver cells in immunodeficient NOD/SCID/IL-2Rγ −/− (NSG) mice 254 .The authors observed that these mice generated functional leukocytes, T-cells, natural killer cells, and monocyte/macrophages at 12 weeks post-engraftment 254 .These mice were infected intranasally with M. tuberculosis, and the infection resulted in organized granulomatous lesions, caseous necrosis, bronchial obstruction, and crystallization of cholesterol deposits at the site of infection, indicating pathology similar to human disease.Moreover, human leukocytes showed migration to the spleen, lung, and liver, and functional CD4 + and CD8 + T-cell subsets were observed in these tissues 254 .Lee et al. also generated BLT mice by transplantation of human fetal liver, thymus, and hematopoietic stem cells in severely immunodeficient NSG mice 255 .The authors showed that BCG-infected NSG mice generated a higher number of IFN-γ producing cells.However, despite increased numbers of IFN-γ producing cells, the transgenic mice were susceptible to infection with BCG 255 .Angelo et al. used HuMurine's humanized NOG (Hu-M TM ) mice to assess the efficacy of standard BCG vaccine or a vaccine containing CpG-C adjuvant, an ESAT-6-based liposomal formulation 256 .The authors demonstrated that the immune responses generated by the humanized mice were comparable to those observed in C57BL/6 mice and guinea pigs.The immunization of humanized mice resulted in the induction of human CD4 + and CD8 + T-cells and the expression of various cytokines such as IFN-γ, IL-2, and TNF-α, which are known to confer better protection against M. tuberculosis.Collectively, results obtained with humanized mice is similar to the data observed in C57BL/6 mice and guinea pigs, suggesting immunization with vaccine can induce T-cell response in humanized mice 256 .Many mycobacterial cell wall lipids are known to be presented by nonpolymorphic human CD1 molecules, resulting in the stimulation of unconventional T-cell subsets 257 .Zhao et al., have developed a transgenic mice model that expresses proteins such as group 1 CD1 molecules (hCD1Tg) and a CD1b-restricted, mycolic-acid specific TCR (DN1Tg) that are necessary for the presentation of lipid antigens to T-cells 258 .The authors showed that aerosol M. tuberculosis infection of these mice resulted in the generation of CD-1-restricted M. tuberculosis lipidspecific activated polyfunctional T-cells.In addition, the adoptive transfer of DN1 T-cells in hCD1Tg/Rag −/− mice prior to the M. tuberculosis challenge resulted in reduced viable bacterial counts by ~5.0, 10.0, and 10.0-fold in lungs, spleens, and liver, respectively, compared to mice that received no DN1 T-cell transfer 258 .Immunization of humanized mice with an adenovirus-based AdHu5Ag85A resulted in the generation of CD4 + T-cell response 259 .Further, the polyfunctional properties of CD4 + T-cells from humanized mice were comparable to the response observed in participants enrolled in a Phase I clinical study 259,260 .Taken together, the humanized mice model offers a promising surrogate model for assessing the effectiveness of potential vaccines before proceeding to clinical trials.Zebrafish model of infection M. marinum infection of zebrafish is emerging as a model to study TB infections.Mycobacterial infection in humans and zebrafish shows similar latent infection features and spontaneous disease reactivation 261 .The zebrafish model of infection offers several advantages in laboratory settings.Firstly, adult zebrafish and embryos can be easily infected.Secondly, the transparency of zebrafish embryos allows for the use of advanced optical techniques, leading to more profound insights into the infection dynamics.Thirdly, genetic manipulations in zebrafish are relatively easy, and infection with M. marinum results in macrophage recruitment.Zebrafish and humans share a common locus for pro-and anti-inflammatory cytokine regulation 262 .Zebrafish have been used as a model for TB vaccine evaluation, and immunization of zebrafish with BCG resulted in improved survival rate compared to saline control against both low and high doses of M. marinum infection 263,264 .In another study, immunization of zebrafish with an attenuated M. marinum strain (L1D) harboring a deletion in the PE/PGRS family of proteins (MAG 24-1) was able to impart protection against challenge with the virulent M. marinum OSU-214 strain.The authors observed a significantly improved fish survival rate compared to vaccination with culture filtrate proteins (CFP) or heat-killed OSU-214 strain or control group 265,266 .Intramuscular immunization of zebrafish with DNA vaccines encoding Ag85C, CFP-10, and ESAT-6 (pCMV-Ag85B/CFP-10/ESAT-6) resulted in fewer granulomas and reduced mycobacterial dissemination following low dose M. marinum infection 263 .In addition, immunization with DNA vaccines resulted in a higher survival rate of zebrafish infected with high doses of M. marinum when compared to sham immunized control 263 .The observed protection was dependent on cell-mediated adaptive immune response as immunization with pCMV-Ag85B/CFP-10/ESAT-6 was unable to enhance the survival rate of Rag1 (recombination activation protein 1) deficient zebrafish that lack functional T-and Blymphocytes 263 .In another study, immunization of zebrafish with a DNA vaccine overexpressing Ag85B, ESAT-6, and RpfE enhanced the protective efficacy imparted by BCG and the survival rate 264 .To identify new antigen candidates using the zebrafish model, 15 M. marinum antigens that showed differential expression during the mycobacterial infection cycle were assessed as DNA vaccine candidates in both low and high doses of M. marinum infection 261 .In a low-dose infection model, immunization of zebrafish with DNA vaccines encoding RpfE, PE5_1, PE31, and Cdh (M.tuberculosis homologs include RpfE/ Rv2450c, PE15/Rv1386, PE13, and Rv2289, respectively) reduced the bacterial burden by 50-88% compared to zebrafish immunized with the plasmid vector 261 .However, only the DNA vaccine expressing RpfE significantly improved the survival rate of zebrafishes compared to controls in a high-dose infection model 261 .In agreement, subcutaneous immunization of mice with RpfE protein has been shown to confer a high level of protection against M. tuberculosis challenge 95 .The authors observed 100.0-fold and 10.0-fold CFU reduction in vaccinated animals' lungs and spleens, respectively, compared to sham-immunized mice.The same 15 differentially expressed antigens were also used to identify post-exposure protective antigens in a zebrafish dexamethasoneinduced reactivation model following M. marinum infection 267 .The authors showed that RpfB and MMAR_4207 protect TB reactivation when evaluated as a post-exposure DNA vaccine in an immunosuppression-based reactivation model of zebrafish.Among Rpf homologs, M. tuberculosis RpfB has emerged as an immunogenic and promising vaccine candidate antigen 12,96,97,99 .Taken together, zebrafish has emerged as a useful model to (i) study functions of the innate and adaptive immune response, (ii) study latency, dormancy, and disease reactivation, and (iii) for preclinical evaluation of potential vaccine candidates 268 .

Conclusion
New and more effective vaccines are urgently needed to eradicate TB.In view of the fact that only a small portion of the M. tuberculosis antigenome has been explored for designing better TB vaccination strategies, innovation in antigen discovery is a crucial aspect of TB research.BCG, although prevents disseminated TB in children, its major drawback is the variable efficacy against pulmonary TB in adults and limited effect on TB transmission 269 .Better protective vaccines are needed to complement or substitute BCG.Identification of antigens that induce protective responses that correlate with natural protection to M. tuberculosis infection, disease or disease recurrence in humans is critical for vaccine design.New protective antigens are needed, also in view of the failure of MVA85A in BCGvaccinated infants 196 .To date, a range of approaches have been used to identify many promising vaccine-candidate antigens and epitopes.Traditional methods of identification of immunogenic proteins in the pregenomic era involved isolation and biochemical characterization of M. tuberculosis proteins expressed during in vitro culture.Advancements in antigen discovery approaches have helped improve our understanding of the M. tuberculosis antigenome and increased the flux of candidate vaccines in preclinical testing.Over the last two decades, many potentially protective M. tuberculosis antigens have been discovered using genome-wide approaches and bioinformatics tools.However, only a handful of antigens have been evaluated as candidate vaccine targets in preclinical and clinical settings with variable success in protecting against TB compared to BCG.In addition to secretory antigens, dormancy-associated antigens and resuscitation-promoting factors have emerged as promising targets for development of TB vaccination strategies especially considering the inability of BCG to induce T-cell responses against dormancy antigens 270,271 .One factor that has hindered the identification of protective antigens is the complexity of the immune response against M. tuberculosis.Immunological readouts focused on IFN-γ production may not comprehensively capture the M. tuberculosis antigenome, and identification of antigens that trigger unconventional T-cell responses may be necessary for designing TB vaccines equipped to trigger a wider range of immune responses against M. tuberculosis 272 .While crucial role of cell-mediated immunity in protection against TB has been established, recent identification of naturally occurring antibodies that are protective against M. tuberculosis infection in humans suggests that antibody-mediated immunity may also play an important role in designing future vaccines against TB 180 .To propel vaccine candidates in TB vaccine pipeline, it is crucial to evaluate their immunogenicity and protective efficacy in animal models of relevance to human TB disease.Since a single animal model is unable to fully recapitulate the complexity of human disease, there is a need to evaluate the effectiveness of TB vaccine candidates in multiple preclinical models to predict their efficacy in humans.The existing animal models, such as mice, guinea pigs, and NHP, have been useful to evaluate the safety, immunogenicity, and protective efficacy of potential vaccine candidates.These models are helpful, but more preclinical animal models and in vitro models that can recapitulate discerning features of human TB disease are required.The recently reported diversity outbred (DO), collaborative cross (CC) and ultra-low dose (ULD) mouse models offer a better choice over conventional models for investigating the influence of host genetics on disease outcome and protection imparted by BCG.The humanized mice and organoid models provide an advantage in studying human-like granuloma and immune response.In addition, the human challenge model would facilitate the TB vaccine development pipeline and would aid in 1) the selection of vaccine candidates for efficacy trials, 2) optimizing dose and route of immunization, and 3) the identification of immune correlates of protection.Although there have been significant advancements in TB vaccine research there are still challenges in designing effective TB vaccines.These include an incomplete understanding of the pathogenicity of M. tuberculosis, lack of correlates of protection, and absence of an appropriate animal model to evaluate vaccines.Despite these challenges, immunization of non-human primates with RhCMV/TB and IV BCG has shown sterilizing immunity in a proportion of vaccinated animals and has renewed enthusiasm for TB vaccine research 11,13 .Intravenous administration of BCG was found to induce superior protection than intradermal or aerosol delivery, as observed by reduction in mycobacterial growth, pathology and granuloma formation in various tissues, with no detectable M. tuberculosis infection in six out of ten macaques receiving IV BCG 13 .The T-cell response induced by intravenous BCG immunization was mostly of Th1 type with some contribution of Th17 type.This unprecedented protection against M. tuberculosis challenge conferred by IV BCG administration suggests that the efficacy of BCG itself can be improved by appropriate route of administration and argues against the notion that BCG lacks antigens required for eliciting protective immune response and that protective antigens are critical for designing effective vaccination strategies against TB.However, transient splenomegaly was observed following intravenous administration of BCG in NHPs and it remains to be seen how this may pose a hindrance to clinical studies in humans.Presently, given the success of two vaccine candidates in NHP models and promising proof-of-concept phase IIb efficacy trial of M72/AS01E in HIV-negative adults with LTBI, it is imperative to prioritize the identification of correlates of protection relevant to the human population that can be further validated in clinical trials.

Fig. 2 |
Fig. 2 | Animal models used in TB research.This figure highlights various animal models in TB vaccine development research and the advantages and disadvantages of each animal model.This figure was generated using BioRender.

Table 1 |
Details of TB vaccine candidates in different stages of clinical trials Live attenuated / Prime A M. tuberculosis mutant with deletion mutations in the virulence genes phoP and fadD26 encoding two major virulence factors.1.Subcutaneous immunization of C57BL/6 mice with MTBVAC resulted in ～4.0-5.0-foldbetter protection in the lungs compared to immunization with BCG. 2. Studies in SCID mice showed comparable safety profiles as BCG. 3.
1. ∼200-fold reduction in bacterial burdens in lungs in latent infection model of guinea pigs compared to the saline group 2. In a spontaneous M. tuberculosis relapse model, mice receiving chemotherapy and AEC/BCO2 displayed significantly lower bacterial load in the lungs and spleen (4.0-20.0-foldreduction) compared to chemotherapy-only group.Lu et al., 2015, Guo et al., 2022, Lu et al., 2022.

Table 1 (
continued) | Details of TB vaccine candidates in different stages of clinical trials Prophylactic immunization of guinea pigs with MIP resulted in 10.0-fold reduced bacterial loads compared to BCG immunized animals.4. MIP given as a prophylactic booster to BCG significantly reduced the lungs and splenic bacterial burdens post M. tuberculosis challenge in guinea pigs and mice compared to BCG immunized group.

Table 1 (
continued) | Details of TB vaccine candidates in different stages of clinical trials Boosting of BCG with GamTBvac resulted in comparable protection to BCG alone in mice and guinea pigs infected with M. tuberculosis via intravenous or aerosol route.2.Prime-boost immunization significantly enhanced the survival of guinea pigs infected with M. tuberculosis than BCG alone.Fig. 1 | Summary of TB vaccine candidates in clinical development pipeline.Globally currently 17 vaccine candidates are being evaluated in different stages of clinical development.These candidate vaccines can be classified into five categories: viral vector based, subunit vaccines, live attenuated, mRNA or whole cell vaccines from other strains of Mycobacterium.These vaccines are currently being evaluated in either Phase I or II or III.